FastQCFastQC Report
Tue 7 Feb 2017
MiSeqF.fq

Summary

[OK]Basic Statistics

MeasureValue
FilenameMiSeqF.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences799567
Sequences flagged as poor quality0
Sequence length40-250
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAGATA6150.001082774113.8852219
GCGTGTA1300.001644217913.139199170-174
GTCCCCA2200.0079272948.872115130-134
GATCGCG3450.0051034087.0719767115-119
Produced by FastQC (version 0.11.4)